Successful liver-directed gene delivery by ERCP-guided hydrodynamic injection (with videos)
نویسندگان
چکیده
منابع مشابه
Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver
Development of a safe and effective method for gene delivery to hepatocytes is a critical step toward gene therapy for liver diseases. Here, we assessed the parameters for gene delivery to the livers of large animals (pigs, 40-65 kg) using an image-guided hydrodynamics-based procedure that involves image-guided catheter insertion into the lobular hepatic vein and hydrodynamic injection of repor...
متن کاملAdenovector Mediated Inflammation in Mice Liver Boosted by Hydrodynamic Injection
Background and Aims: Adenovector gene transfer induces inflammatory response that finally leads to vector removal from delivered site. The effect of hydrodynamic pre-injection on Adenovector mediated liver inflammation remained elusive. Materials and Methods: Different mice groups were pre-treated by hydrodynamic saline, dexamethasone or nothing prior to Adeno-Luciferase administration. Their s...
متن کاملImage-Guided Hydrodynamic Gene Delivery: Current Status and Future Directions
Hydrodynamics-based delivery has been used as an experimental tool to express transgene in small animals. This in vivo gene transfer method is useful for functional analysis of genetic elements, therapeutic effect of oligonucleotides, and cancer cells to establish the metastatic cancer animal model for experimental research. Recent progress in the development of image-guided procedure for hydro...
متن کاملEffects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery
Hydrodynamic gene delivery is a common method for gene transfer to the liver of small animals, and its clinical applicability in large animals has been demonstrated. Previous studies focused on functional analyses of therapeutic genes in animals with normal livers and little, however, is known regarding its effectiveness and safety in animals with liver fibrosis. Therefore, this study aimed to ...
متن کاملSafety Assessment of Liver-Targeted Hydrodynamic Gene Delivery in Dogs
Evidence in support of safety of a gene delivery procedure is essential toward gene therapy. Previous studies using the hydrodynamics-based procedure primarily focus on gene delivery efficiency or gene function analysis in mice. The current study focuses on an assessment of the safety of computer-controlled and liver-targeted hydrodynamic gene delivery in dogs as the first step toward hydrodyna...
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ژورنال
عنوان ژورنال: Gastrointestinal Endoscopy
سال: 2018
ISSN: 0016-5107
DOI: 10.1016/j.gie.2018.06.022